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Journal of Molecular Biology 98, 503–517. .
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  • Finally, they separated the fragments using gel electrophoresis, a technique developed in the 1960s and still commonly used as a way to sort nucleic acid molecules of different sizes (Figure 1.
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    Gel electrophoresis also removes enzymes and salts that were present in the digestion reactions.

  • Gel electrophoresis is a technique used to separate DNA fragments (or other macromolecules, such as RNA and proteins) based on their size and charge.
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    To provide a detailed analysis of agarose gel electrophoresis results after restriction enzyme cutting, it would be helpful to have specific information about the experiment, such as the type of DNA being analyzed, the restriction enzyme used, and the specific objective of the experiment.

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  • Regardless of whether a single or double restriction digest is done, the 5′ phosphate groups of the vector must be removed, if restriction digest provides identical termini, in order to prevent self-ligation (i.
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